Accumulated intermediates, in the presence of reduced glutathione, are transported to the vacuoles, whereupon the development of the red color. Spectramax microplate spectrophotometer operators manual. For compounds with a known extinction coefficient, concentration can be calculated directly from absorbance. I used spectramax m3 plate reader to measure the fluorescence change through time. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Spectramax plus384 is the only spectrophotometer with a builtin cuvette port and microplate drawer. Combine pdfs in the order you want with the easiest pdf merger available. Microplate readers, multimode, spectramax m series vwr. Spectramax i3 spectratest lm1 protocol evelyn mcgown 20726 16. Merge, split, compress, convert, rotate, unlock and watermark pdfs with just a few clicks. The molecular devices spectramax m5m5e microplate reader was tested for compatibility with life technologies geneblazer assays. Separate one page or a whole set for easy conversion into independent pdf files. Spectramax i3 spectratest fl1 protocol evelyn mcgown 20726 16. Insert your plate into the blue adapter, making sure it is lying flat inside the clips.
The spectramax m3, m4, m5, and m5 e microplate readers are a series of dualmonochromator, multidetection, multimode instruments with a triplemode cuvette port and 6well to 384well microplate reading capability. Molecular devices spectramax plus 384 uv absorbance microplate reader harlow scientific. Microplate readers laboratory equipment laboratory. In addition, the id5 reader measures trf and fp and can be expanded to include trfret, htrf, bret, dual luciferase reporter assays with injectors, and western blot detection. Luciferase detection instrumentation guide thermo fisher. Spectramax id3 multimode microplate reader molecular. Spectramax m3 multimode microplate reader, see page 202. The following document is intended to demonstrate setup of this instrument. Life sciencemolecular devices spectramax 384plus from delhi.
The spectramax i3x multimode detection platform utilizes a patentpending design that allows for realtime system configuration by the user in less than two minutes. One of our customer services cs requirement was to be able to retrieve from m3 a combined. Spectramax m3 microplate reader from molecular devices. Spectratest lm1 this experiment contains this introduction, the certificate of calibration information certinfo, revision information. Molecular devices versamax microplate reader the molecular devices versamax elisa microplate reader enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequencefree assay flexibility. Explore more about the spectramax i3x multimode plate reader, a userupgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Spectramax m series multimode microplate readers biothing.
Dec 03, 20 the spectramax minimax 300 imaging cytometer enables cell visualization and cellbased analysis on the spectramax i3 multimode microplate reader. Accumulated intermediates, in the presence of reduced glutathione, are transported to the vacuoles, whereupon the development of the. This dual monochromatic, multi mode microplate reader delivers three modes of detection, uv vis absorbance reading, fluorescence and luminescence, and a broad spectrum of formats from cuvettes to 6, 12, 24, 48, 96, and 384well microplates. Merge allows you to load objects from saved scene files into the current scene. Choose from a three m3, four m4, or five m5m5e mode reader customized to your specific applications or budgetary needs, while optional capabilities. Detection modes include absorbance uvvis abs and fluorescence intensity fi and have optical performance comparable to a. You can also use merge to combine an entire scene with another. The spectramax m series multimode microplate readers measure uv and visible absorbance, fluorescence, luminescence, fluorescence polarization, trf and htrf. The spectramax m5 multimode microplate reader is owned by the nadler lab and available to all investigators through user fees this reader does uv and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes, standard. The versamax microplate reader is built around a grating monochromator. Spectramax m3 multimode microplate reader, molecular devices.
Key features upgradeable platform for changing lab needs threemode cuvette port for assay development. With optimized reagents and the industryleading data acquisition and analysis tool softmax pro 7 software, the spectramax id3 allows customization of workflow to address challenging research applications. Spectramax plus 384 microplate spectrophotometer operators manual iii this warranty only covers defects arising under normal usage and does not cover malfunctions of failures resulting from misuse, abuse, neglect, alteration, modification, or repairs by other than an authorized molecular devices corporation service facility. Spectramax plus 384 microplate spectrophotometer operator s manual iii this warranty only covers defects arising under normal usage and does not cover malfunctions of failures resulting from misuse, abuse, neglect, alteration, modification, or repairs by other than an authorized molecular devices corporation service facility. The spectramax m3 multimode microplate reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform.
Spectramax m3, m4, m5, and m5e multimode microplate reader. Spectramax m2 readers provide for easy conversion and optimization of verylowthroughput to mediumhighthroughput assays, faster, more precise results and reagent savings. Molecular devices spectramax id3 multimode microplate reader. Robust, highvalue microplate readers can run samples based on predefined protocols and standard filter modes cover the entire visible range for a variety of assays. Endpoint, kinetic, spectrum, and multi point well scanning applications combining. Spectramax plus 384 microplate reader manual conquer.
Spectramax m3 laboratory equipment pdf manual download. Molecular devices spectramax id3 multimode microplate. Spectramax m3, m4, m5, and m5e multimode microplate. Spectramax i3 spectratest lm1 protocol softmax pro. Choose from a three m3, four m4, or five m5m5e mode reader. Molecular devices spectramax m2 multimode microplate reader. Detection modes include absorbance uvvis abs and fluorescence intensity fi and have optical performance comparable to. All spectramax m series multimode microplate readers measure uv and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes as standard. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read.
Spectramax m2 is a dualmonochromator, multidetection microplate reader with a dualmode cuvette port and 6384 microplate reading capability. Ten to thousand time diluted samples were used in the assay according to the manufacturers instructions. Spectramax i3 spectratest fl1 protocol softmax pro. The spectramax i3 x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The spectramax m3, m4, m5, and m5 e microplate readers are a series of dualmonochromator, multidetection, multimode instruments with a triplemode cuvette port and 6well to 384well microplate reading. Molecular devices versamax microplate reader marshall.
Spectramax and versamax plate readers operators manual 01120126 rev a 5 1. Detection modalities include absorbance uvvis abs and fluorescence intensity fi and have optical performance comparable to a topoftheline dedicated spectrofluorometer or spectrophotometer. Uvvisible absorbance abs fluorescence intensity fi glow luminescence lumi the. The optical performance is comparable to a topoftheline dedicated. Molecular devices spectramax plus384 microplate reader manual attachments molecular devices spectramax plus384 microplate reader manual 624 kb. Apr 07, 2015 molecular devices spectramax plus 384 uv absorbance microplate reader harlow scientific. Softmax pro software has been integrated by many leading robotics and. All m series readers accept cuvettes and 6, 12, 24, 48, 96, and 384well microplates.
In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command. Molecular devices just launched spectramax id3 multimode microplate reader is the cornerstone of complete laboratory solution helping to expand the boundaries of research. Spectramax m series multimode microplate readers 7 the spectradrop microvolume microplate offers the ability to use as little as 2l samples with 24 or 64well plates. Spectramax i3 multimode detection platform youtube. Spectratest fl1 this experiment contains this introduction, the certificate of calibration information certinfo, revision information.
We therefore gathered salient information about many of the instruments available to date. Softmax pro data acquisition and analysis software. Spectramax plate reader eastern virginia medical school. Spectramax m3 microplate reader from molecular devices llc description the spectramax m3 multimode microplate reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Spectramax m3, m4, m5, and m5 e multimode microplate readers. Spectramax m3 multimode microplate reader from molecular. This easy to use multimode plate reader is designed to handle the work flow of multiple research projects.
While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Setup guide on the molecular devices spectramax m5m5e. The colorimetric peroxidase substrate tetramethylbenzidine was quantified by measurement of absorbance at 450 and 540 nm spectramax m3, molecular devices. Spectramax plus 384 microplate reader a microplate spectrophotometer with a builtin cuvette port the pathcheck sensor also allows for the elimination of standard curves.
This revolution in modularity makes nocompromise detection the norm. This document is in pdf format and can be viewed using a pdf viewer like adobe. The spectramax m3, m4, m5 and m5e are modular, upgradeable dualmonochromator microplate reader platforms that allow you to upgrade with other detection modes at a later time. Superior flexibility the spectramax i3 multimode platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The spectramax i3 platform from molecular devices is a multimode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. The molecular devices spectramax m3 multimode microplate reader covers a wide range of applications without the need to change filters. Compatible automation solutions for spectramax readers. In these mutants, intermediate metabolites of adenine biosynthesis pathway are accumulated. The molecular devices spectramax m2 is a dualmonochromator, multidetection microplate reader with a dualmode cuvette port and 6384 microplate reading capability. Turn on the plate reader with the rocking toggle switch on the back righthand side. The spectramax m series multimode microplate readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Spectramax m series multimode microplate readers from. Located at the right front of the spectramax plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Save as pdf to save the printed output of a file to a portable document format.
Description the onboard microprocessor calculates and reports the absorbance, % transmittance, andor fluorescence for each well of a microplate or from the cuvette port. The spectramax m3 multimode microplate reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Molecular devices spectramax m3 multi mode microplate reader. Spectramax m series multimode microplate readers molecular. The spectramax id3 and id5 multimode microplate readers measure absorbance, fluorescence, and luminescence. Spectramax m2m2e user guide university of michigan. The broad array of spectramax i3x detection cartridges enables the user. Oral administration of heattreated lactobacilli modifies.
With optimized reagents, option to operate the readers. These settings are also valid for the spectramax m3m4, flexstation 3 multimode. Type the name of the file to which you want to append this file. The spectramax id3 multimode microplate reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The cuvette chamber spectramax plus 384 only figure 1. Every tool you need to use pdfs, at your fingertips.
Flexible throughput read one sample or up to 384 at a time its your choice. M5m5e mode reader customized to your specific applications or budgetary needs, while optional capabilities. Acinterlab provides quality, brandname equipment, reagents and supplies to laboratories across the world. Spectramax plus 384 microplate reader molecular devices. Future ready the spectramax i3 x multimode microplate reader measures spectralbased absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Spectramax id3 multimode microplate reader the spectramax id3 multimode microplate reader is the cornerstone of a. With optimized reagents and the industryleading data acquisition and analysis tool, softmax pro 7 software, the spectramax to perfectly match your needs. Now you can run both standard spectrophotometer and microplate reader applications on the same instrument. The spectramax m3, m4, m5, and m5e microplate readers are a series. Group sections enable you to combine the wells in a plate or several cuvettes into groups.